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Research Open-source Bond Lab Reactors

 

The Bond Lab had developed a batch-fed bioelectrochemical system for studying pure and co-cultures of bacteria using relatively small, but well-defined electrodes. In cooperation with the Bond Lab, we have developed an open-source design package with the goal of allowing anyone to independently build these bioelectrochemical systems. We are providing the documents listed below to assist with your efforts to build these reactors.


Documents available

       Bond Lab Reactor Manual. This describes details on the reactors, how to fabricate them, and materials necessary.

       Design drawings. This is a PDF document showing the necessary information for a machinist to be able to copy the design without referring to the source CAD file. New: electrode caps for reference electrode housing.

       Parts list. This is a detailed document listing all items necessary to make the reactors. It includes vendor, package quantity, part descriptions, and quantities for single and triplicate reactor setups.

       Quotes. Includes quotes from vendors made at the time. Note – prices will vary with time and order quantity.

       Open-source reference electrode housing. CAD drawing to manufacture reference electrodes, which are made out of glass with a ceramic frit.

 

Reactor description

This reactor was originally designed and used by the Bond Lab (Daniel Bond, U. Minnesota) for the study of microbial electrochemistry in an effort to minimize oxygen diffusion into the reactor headspace. These reactors feature the following:

       15-mL internal volume (can be lower depending on electrode choice)

       Typical electrode sizes <1cm2

       3 ports for electrodes (working, counter, and reference)

       2 ports for gas (in and out)

       1 port for sampling and inoculation

       Autoclavable

       Can be operated as a CSTR with temperature control

       Customizeable potential for additional ports to allow continuous flow


Example reactor photos